Claudin 5 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10296R-RBITC |
| Product Name | Claudin 5 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Claudin 5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7122 |
| Swiss Prot | O00501 |
| Subcellular location | Cell membrane |
| Synonyms | AWAL; BEC 1; BEC1; Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome); Claudin5; Claudin-5;CLDN 5; CLDN5; CPETR L1; CPETRL 1; CPETRL1; TMDVCF; TMVCF; Transmembrane protein deleted in VCFS; Transmembrane protein deleted in velocardiofacial syndrome; Androgen withdrawal and apoptosis induced protein RVP1 like; CLD5_HUMAN. |
| Background | Plays a major role in tight junction-specific obliteration of the intercellular space.This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively, spliced __transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | FCM1:20-100 |
Frequently asked questions
Which applications is Claudin 5 Polyclonal Antibody, RBITC Conjugated (bs-10296R-RBITC) validated for, and at what dilution?
bs-10296R-RBITC is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Claudin 5 Polyclonal Antibody, RBITC Conjugated (bs-10296R-RBITC) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-10296R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Claudin 5 Polyclonal Antibody, RBITC Conjugated (bs-10296R-RBITC)?
KLH conjugated synthetic peptide derived from human Claudin 5
What are the excitation and emission wavelengths of the RBITC conjugate (bs-10296R-RBITC)?
RBITC on bs-10296R-RBITC: excitation / emission 540nm/625nm.