LXR alpha Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10311R-PerCP-Cy5.5 |
| Product Name | LXR alpha Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Horse, Chicken |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LXR alpha |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10062, 7376 |
| Subcellular location | Nucleus |
| Synonyms | Oxysterols receptor LXR-beta; Liver X receptor beta; Nuclear receptor NER; Nuclear receptor subfamily 1 group H member 2; NR1H2; LXRB; NER; UNR; Liver X receptor alpha; Nuclear receptor subfamily 1 group H member 3; Oxysterols receptor LXR-alpha; NR1H3; LXRA |
| Background | The LXR receptors (including LXR-_ and LXR-_) bind oxysterols and play key roles in maintaining cholesterol homeostasis in macrophages, primarily by regulating multiple components of the reverse cholesterol transport. They also are potent inhibitors of inflammation and are capable of repressing cytokine and chemokine production by Toll-like receptor (TLR)-activated macrophages. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is LXR alpha Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-10311R-PERCP-CY5-5) validated for, and at what dilution?
BS-10311R-PERCP-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LXR alpha Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-10311R-PERCP-CY5-5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Horse, Chicken.
How should BS-10311R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LXR alpha Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-10311R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from human LXR alpha
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-10311R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-10311R-PERCP-CY5-5: excitation / emission 482nm/694nm.