Help

VNN1 Polyclonal Antibody, APC-Cy7 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Sheep
  • Rabbit
Overview
Catalog # bs-10314R-APC-Cy7
Product Name VNN1 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Sheep, Rabbit
Specifications
Conjugation APC-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human VNN1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8876
Swiss Prot O95497
Subcellular location Cell membrane
Synonyms HDLCQ8; High density lipoprotein cholesterol level quantitative trait locus 8, included; Pantetheinase; Pantetheine hydrolase; Tiff66; V-1 antibody Vanin 1; Vanin-1; Vannin 1; Vascular non inflammatory molecule 1; Vascular non-inflammatory molecule 1; VNN 1; VNN1; VNN1_HUMAN.
Background Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is VNN1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-10314R-APC-Cy7) validated for, and at what dilution?

bs-10314R-APC-Cy7 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does VNN1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-10314R-APC-Cy7) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Rabbit.

How should bs-10314R-APC-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise VNN1 Polyclonal Antibody, APC-Cy7 Conjugated (bs-10314R-APC-Cy7)?

KLH conjugated synthetic peptide derived from human VNN1

What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-10314R-APC-Cy7)?

APC-Cy7 on bs-10314R-APC-Cy7: excitation / emission 650nm/780nm.