Phospho-HDAC4 (Ser246) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-10325r-cy3-100uL |
| Product Name | Phospho-HDAC4 (Ser246) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human HDAC4 around the phosphorylation site of Ser246 |
| Modification Site | Ser246 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9759 |
| Subcellular location | Secreted |
| Synonyms | HDAC4Ser246; HDAC4S246; p-HDAC4Ser246; p-HDAC4S246; HD 4; HD4; HDAC 4; HDAC A; HDACA; Histone Deacetylase 4; Histone Deacetylase A; KIAA0288; EC 3.5.1.98; HA6116; HDA4_CAEEL; Histone deacetylase 4; CeHDA-7; Histone deacetylase 7. |
| Background | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is Phospho-HDAC4 (Ser246) Polyclonal Antibody, Cy3 Conjugated (bs-10325R-Cy3) validated for, and at what dilution?
bs-10325R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-HDAC4 (Ser246) Polyclonal Antibody, Cy3 Conjugated (bs-10325R-Cy3) react with?
Tested reactivity: Human.
How should bs-10325R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-HDAC4 (Ser246) Polyclonal Antibody, Cy3 Conjugated (bs-10325R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human HDAC4 around the phosphorylation site of Ser246
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-10325R-Cy3)?
Cy3 on bs-10325R-Cy3: excitation / emission 512,550nm/570,615nm.