Factor I heavy chain Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10339R-PE |
| Product Name | Factor I heavy chain Polyclonal Antibody, PE Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Complement factor I heavy chain |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | Factor I heavy chain; AHUS3; C3b INA; C3b inactivator; C3B/C4B inactivator; C3BINA; CFAI_HUMAN; CFI; Complement component I; Complement control protein factor I; Complement factor I; Complement factor I heavy chain; F1; factor I; FactorI; FI; I factor; ; KAF; Konglutinogen activating factor; Heavy chain of factor I; OTTHUMP00000219728. |
| Background | The complement pathway is an important host defense system that contributes to both innate and acquired immunity. There are three pathways of complement activation: the classical pathway, lectin pathway and alternative pathway. Complement protein Factor I is a key serine protease that modulates the complement cascade by regulating the levels of C3 convertases. It circulates in plasma as a heavily N-glycosylated heterodimer made up of two disulfide linked chains, each carrying three N-linked oligosaccharide chains that may have both structural and functional roles in the interactions with the natural substrate and the cofactor during catalysis. Factor I is a serine protease with a high degree of specificity for C3b and C4b. It requires protein cofactors for cleavage of these complement proteins; Factor H, CR1 or MCP are required for C3b cleavage, and C4bp or CR1 are required for C4b cleavage. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is Factor I heavy chain Polyclonal Antibody, PE Conjugated (bs-10339R-PE) validated for, and at what dilution?
bs-10339R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Factor I heavy chain Polyclonal Antibody, PE Conjugated (bs-10339R-PE) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bs-10339R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Factor I heavy chain Polyclonal Antibody, PE Conjugated (bs-10339R-PE)?
KLH conjugated synthetic peptide derived from human Complement factor I heavy chain
What are the excitation and emission wavelengths of the PE conjugate (bs-10339R-PE)?
PE on bs-10339R-PE: excitation / emission 496,564nm/578nm.