Phospho-Calcineurin B (Tyr106) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10342R-Cy3-100uL |
| Product Name | Phospho-Calcineurin B (Tyr106) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Zebrafish |
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit, Guinea Pig, Drosophila |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated syntheticphosphopeptide derived from human Calcineurin B around the phosphorylation site of Tyr106 |
| Modification Site | Tyr106 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5534 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Calcineurin B phospho Y106; Calcineurin B phospho Tyr106; p-Calcineurin B Tyr106; Calcineurin subunit B type 1; CALNB1; CANB1_HUMAN; Cna2; CNB; CNB1; OTTHUMP00000201960; OTTHUMP00000201961; Ppp3r1; PPP3R1 protein phosphatase 3 formerly 2B, regulatory subunit B, alpha isoform; alpha isoform calcineurin B, type I; calcineurin B, type I 19kDa; protein phosphatase3 formerly2B, regulatory subunit B, alpha isoform antibody Protein phosphatase 2B regulatory subunit 1; Protein phosphatase 2B regulatory subunit B alpha; protein phosphatase 3 formerly 2B, regulatory subunit B, 19kDa, alpha isoform calcineurin B, type I; Protein phosphatase 3 regulatory subunit B alpha; Protein phosphatase 3 regulatory subunit B alpha isoform 1. |
| Background | In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunit have been identified, designated PP1, PP2A, PP2B and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4), is a putative member of a novel PP family. The PP2B family comprises subfamily members PP2B-A alpha, PP2B-A Beta and PP2B-A Gamma. Two additional regulatory subunits been identified, designated PP2B-B1 and PP2B-B2. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-Calcineurin B (Tyr106) Polyclonal Antibody, Cy3 Conjugated (bs-10342R-Cy3) validated for, and at what dilution?
bs-10342R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Calcineurin B (Tyr106) Polyclonal Antibody, Cy3 Conjugated (bs-10342R-Cy3) react with?
Tested reactivity: Zebrafish. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit, Guinea Pig, Drosophila.
How should bs-10342R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Calcineurin B (Tyr106) Polyclonal Antibody, Cy3 Conjugated (bs-10342R-Cy3)?
KLH conjugated syntheticphosphopeptide derived from human Calcineurin B around the phosphorylation site of Tyr106
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-10342R-Cy3)?
Cy3 on bs-10342R-Cy3: excitation / emission 512,550nm/570,615nm.