phospho-c-ABL (Tyr245) Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10355R-APC |
| Product Name | phospho-c-ABL (Tyr245) Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous to that of Tyr245 in c-ABL isoform IB. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human c-Abl isoform IA around the phosphorylation site of Tyr226 |
| Modification Site | Tyr226 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 25 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | c-Ablphospho Tyr226; c-Ablphospho Tyr226; p-c-AblTyr226; Abelson Murine Leukemia Viral Oncogene Homolog 1; Abelson murine leukemia viral v abl oncogene homolog 1; Abl 1; ABL; Abl protein; Abl1; Bcr/c abl oncogene protein; JTK 7; JTK7; p150; Proto oncogene tyrosine protein kinase ABL1; Transformation gene oncogene ABL; v abl Abelson murine leukemia viral oncogene homolog 1; v abl; ABL1_HUMAN. |
| Background | The c Abl proto oncogene encodes a protein tyrosine kinase that is located in the cytoplasm and nucleus. In chronic myelogenous leukemia and in a subset of acute lymphoblastic leukemias, the c Abl proto oncogene undergoes a (9;22) chromosomal translocation producing a novel rearranged chromosome (the Philadelphia chromosome) As the result of the fusion of c Abl sequences from chromosome 9 to the Bcr gene on chromosome 22. The molecular consequence of this translocation is the generation of a chimeric Bcr/Abl mRNA encoding activated Abl protein tyrosine kinase. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-c-ABL (Tyr245) Polyclonal Antibody, APC Conjugated (bs-10355R-APC) validated for, and at what dilution?
bs-10355R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-c-ABL (Tyr245) Polyclonal Antibody, APC Conjugated (bs-10355R-APC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Pig, Horse, Chicken, Rabbit.
How should bs-10355R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-c-ABL (Tyr245) Polyclonal Antibody, APC Conjugated (bs-10355R-APC)?
KLH conjugated synthetic phosphopeptide derived from human c-Abl isoform IA around the phosphorylation site of Tyr226
What are the excitation and emission wavelengths of the APC conjugate (bs-10355R-APC)?
APC on bs-10355R-APC: excitation / emission 650nm/660nm.