CD86 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-1035r-cy3-100ul |
| Product Name | CD86 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat CD86 |
| Immunogen Range | 140-175/313 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 56822 |
| Swiss Prot | O35531 |
| Subcellular location | Cell membrane |
| Synonyms | B7-2, Cd86 antigen; Cd86; Membrane glycoprotein |
| Background | This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CD86 Polyclonal Antibody, Cy3 Conjugated (bs-1035R-Cy3) validated for, and at what dilution?
bs-1035R-Cy3 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD86 Polyclonal Antibody, Cy3 Conjugated (bs-1035R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-1035R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD86 Polyclonal Antibody, Cy3 Conjugated (bs-1035R-Cy3)?
KLH conjugated synthetic peptide derived from rat CD86 Immunogen range: 140-175/313.
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-1035R-Cy3)?
Cy3 on bs-1035R-Cy3: excitation / emission 512,550nm/570,615nm.