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TPOR Polyclonal Antibody, RBITC Conjugated

Applications

  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Sheep
Overview
Catalog # bs-10362R-RBITC
Product Name TPOR Polyclonal Antibody, RBITC Conjugated
Applications
FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Sheep
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MPL
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4352
Swiss Prot P40238
Subcellular location Cell membrane
Synonyms MPLV; TPOR; C-MPL; CD110; THCYT2; Thrombopoietin receptor; TPO-R; Myeloproliferative leukemia protein; Proto-oncogene c-Mpl; MPL
Background Receptor for thrombopoietin. May represent a regulatory molecule specific for TPO-R-dependent immune responses.
Application Dilution
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is TPOR Polyclonal Antibody, RBITC Conjugated (bs-10362R-RBITC) validated for, and at what dilution?

bs-10362R-RBITC is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TPOR Polyclonal Antibody, RBITC Conjugated (bs-10362R-RBITC) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Sheep.

How should bs-10362R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TPOR Polyclonal Antibody, RBITC Conjugated (bs-10362R-RBITC)?

KLH conjugated synthetic peptide derived from human MPL

What are the excitation and emission wavelengths of the RBITC conjugate (bs-10362R-RBITC)?

RBITC on bs-10362R-RBITC: excitation / emission 540nm/625nm.