Orexin A Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-10376R-RBITC-100uL |
| Product Name | Orexin A Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Orexin A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3060 |
| Synonyms | HCRT 1; HCRT; HCRT1; HCRT2; Hypocretin orexin neuropeptide precursor; Hypocretin 1; Hypocretin 2; Hypocretin A; Hypocretin; Hypocretin neuropeptide precursor; Hypocretin1; NRCLP1; Orexin A; Orexin; Orexin B; Orexin precursor; OrexinA; OX; PPORX; PPOX; PREPROOREXIN; OREX_HUMAN. |
| Background | Orexin A (hypocretin 1) a 33 amino acid peptide and orexin B (hypocretin 2), a 28 amino acid peptide, are both derived from a common 130 amino acid precursor, prepro orexin. Orexin A and Orexin B stimulate food consumption when administered intracerebroventricularly to rats. Orexin gene expression in the brain is highly restricted to distinct populations of neurons located in specific hypothalamic regions, including the lateral hypothalamic area (LHA), a region implicated in feeding behaviour. Orexin A and orexin B bind to and activate two closely related G protein coupled receptors (GPCRs), termed Orexin 1 (OX1) and Orexin 2 (OX2) receptors. Dysfunction of the orexin peptide system has been linked to narcolepsy. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is Orexin A Polyclonal Antibody, RBITC Conjugated (bs-10376R-RBITC) validated for, and at what dilution?
bs-10376R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Orexin A Polyclonal Antibody, RBITC Conjugated (bs-10376R-RBITC) react with?
Tested reactivity: Human.
How should bs-10376R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Orexin A Polyclonal Antibody, RBITC Conjugated (bs-10376R-RBITC)?
KLH conjugated synthetic peptide derived from human Orexin A
What are the excitation and emission wavelengths of the RBITC conjugate (bs-10376R-RBITC)?
RBITC on bs-10376R-RBITC: excitation / emission 540nm/625nm.