F2 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-1037R-BF647-100uL |
| Product Name | F2 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DCP |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2147 |
| Swiss Prot | P00734 |
| Subcellular location | Cytoplasm |
| Synonyms | PT; THPH1; RPRGL2; Prothrombin; Coagulation factor II; F2; Thrombin Activation peptide fragment 1 |
| Background | Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is F2 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-1037R-BF647) validated for, and at what dilution?
bs-1037R-BF647 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does F2 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-1037R-BF647) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-1037R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise F2 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-1037R-BF647)?
KLH conjugated synthetic peptide derived from human DCP
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-1037R-BF647)?
AbBy Fluor-647 on bs-1037R-BF647: excitation / emission 650nm/665nm.