Tenascin C Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1039R-PerCP-Cy5.5-100uL |
| Product Name | Tenascin C Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Pig |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Tn-C |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3371 |
| Swiss Prot | P24821 |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | GP; JI; TN; HXB; GMEM; TN-C; DFNA56; 15-225; Tenascin; Cytotactin; GP 15-225; Glioma-associated-extracellular matrix antigen; Hexabrachion; Myotendinous antigen; Neuronectin; Tenascin-C; TNC |
| Background | Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Tenascin C Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-1039R-PERCP-CY5-5) validated for, and at what dilution?
BS-1039R-PERCP-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Tenascin C Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-1039R-PERCP-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Pig.
How should BS-1039R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Tenascin C Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-1039R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from human Tn-C
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-1039R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-1039R-PERCP-CY5-5: excitation / emission 482nm/694nm.