Chemerin Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-10410R-PerCP |
| Product Name | Chemerin Polyclonal Antibody, PerCP Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat, Rabbit |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat chemerin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 297073 |
| Subcellular location | Secreted |
| Synonyms | Rarres2; TIG2; tazarotene induced gene 2; Retinoic acid receptor responder protein 2; Tazarotene-induced gene 2 protein; RAR-responsive protein TIG2; Chemerin. |
| Background | Chemerin, also known as retinoic acid receptor responder 2 (RARRES2) and tazarotene induced gene 2 (TIG2), is a chemoattractant protein for cells expressing G-protein-linked receptor chemokine-like receptor 1 (CMKLR1, ChemR23 or GPCR-DEZ). The protein is synthesized as a secreted precursor, prochemerin (18 kDa), which is poorly active. Upon proteolytic removal of the last six amino acids, prochemerin is converted into mature active chemerin (15-16 kDa) that acts as a strong and highly specific agonist of its receptor CMKLR1. Chemerin is a newly described adipokine with effects on adipocyte differnetiation and metabolism. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Chemerin Polyclonal Antibody, PerCP Conjugated (bs-10410R-PerCP) validated for, and at what dilution?
bs-10410R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Chemerin Polyclonal Antibody, PerCP Conjugated (bs-10410R-PerCP) react with?
Tested reactivity: Human, Mouse, Rat, Rabbit.
How should bs-10410R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Chemerin Polyclonal Antibody, PerCP Conjugated (bs-10410R-PerCP)?
KLH conjugated synthetic peptide derived from rat chemerin
What are the excitation and emission wavelengths of the PerCP conjugate (bs-10410R-PerCP)?
PerCP on bs-10410R-PerCP: excitation / emission 482nm/677nm.