Cyclooxygenase 2 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10411R-BF350 |
| Product Name | Cyclooxygenase 2 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Cyclooxygenase 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5743 |
| Swiss Prot | P35354 |
| Subcellular location | Cytoplasm |
| Synonyms | COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS; Prostaglandin G/H synthase 2; Cyclooxygenase-2; PHS II; Prostaglandin H2 synthase 2; PGH synthase 2; Prostaglandin-endoperoxide synthase 2; PTGS2 |
| Background | Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Constitutively expressed in some tissues in physiological conditions, such as the endothelium, kidney and brain, and in pathological conditions, such as in cancer. PTGS2 is responsible for production of inflammatory prostaglandins. Up-regulation of PTGS2 is also associated with increased cell adhesion, phenotypic changes, resistance to apoptosis and tumor angiogenesis. In cancer cells, PTGS2 is a key step in the production of prostaglandin E2 (PGE2), which plays important roles in modulating motility, proliferation and resistance to apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Cyclooxygenase 2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-10411R-BF350) validated for, and at what dilution?
bs-10411R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cyclooxygenase 2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-10411R-BF350) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-10411R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cyclooxygenase 2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-10411R-BF350)?
KLH conjugated synthetic peptide derived from human Cyclooxygenase 2
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-10411R-BF350)?
AbBy Fluor-350 on bs-10411R-BF350: excitation / emission 346nm/442nm.