HPV16-E7 Polyclonal Antibody

Applications

  • WB
  • FCM
  • IHC-P
  • IF(IHC-P)

Reactivity

  • Virus
Overview
Catalog # bs-10446R
Product Name HPV16-E7 Polyclonal Antibody
Applications WB, FCM, IHC-P, IF(IHC-P)
Specificity This antibody may also detect HPV35-E7
Reactivity Virus
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from middle of HPV16-E7
Immunogen Range 50-98/98
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Target
Gene ID 1489079
Swiss Prot P03129
Synonyms Protein E7; E7
Background Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Plays also a role in the inhibition of both antiviral and antiproliferative functions of host inteferon alpha.
Application Dilution
WB 1:300-1000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200