phospho-HSP70 (Tyr611) Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10448R-BF405-100uL |
| Product Name | phospho-HSP70 (Tyr611) Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Pig, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human HSP70 around the phosphorylation site of Tyr611 |
| Modification Site | Tyr611 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | HSP70 phospho Tyr611; HSP70 phospho Y611; p-HSP70 Tyr611; p-HSP70 Y611; HSP70; HSP-70; HSP 70; Heat shock 70 kDa protein 1; heat shock 70kDa protein 1A; Heat shock 70kDa protein 1B; Heat shock induced protein; heat shock protein 70; HSP70 1; HSP70 2; HSP70.1; HSP72; HSPA1; HSPA1A; HSPA1B; XXbac BCX40G17.3 001; Heat shock 70 kDa protein 1A/1B; HSP71_HUMAN; Hspa1a; Hsp70-1; HSP72; Hspa1; Hspa1b; Heat shock 70 kDa protein 1A/1B; shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2. |
| Background | This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-HSP70 (Tyr611) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-10448R-BF405) validated for, and at what dilution?
bs-10448R-BF405 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-HSP70 (Tyr611) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-10448R-BF405) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Pig, Rabbit, GuineaPig.
How should bs-10448R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-HSP70 (Tyr611) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-10448R-BF405)?
KLH conjugated synthetic phosphopeptide derived from human HSP70 around the phosphorylation site of Tyr611
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-10448R-BF405)?
AbBy Fluor-405 on bs-10448R-BF405: excitation / emission 401nm/421nm.