Phospho-ITGB2 (Thr758) Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10462R-RBITC-100uL |
| Product Name | Phospho-ITGB2 (Thr758) Polyclonal Antibody, RBITC Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synstic phosphopeptide derived from human CD18 around the phosphorylation site of Thr758 |
| Modification Site | Thr758 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3689 |
| Swiss Prot | P05107 |
| Subcellular location | Cell membrane |
| Synonyms | LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1; Integrin beta-2; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; ITGB2 |
| Background | Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-ITGB2 (Thr758) Polyclonal Antibody, RBITC Conjugated (bs-10462R-RBITC) validated for, and at what dilution?
bs-10462R-RBITC is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-ITGB2 (Thr758) Polyclonal Antibody, RBITC Conjugated (bs-10462R-RBITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, GuineaPig.
How should bs-10462R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-ITGB2 (Thr758) Polyclonal Antibody, RBITC Conjugated (bs-10462R-RBITC)?
KLH conjugated synstic phosphopeptide derived from human CD18 around the phosphorylation site of Thr758
What are the excitation and emission wavelengths of the RBITC conjugate (bs-10462R-RBITC)?
RBITC on bs-10462R-RBITC: excitation / emission 540nm/625nm.