Complement C5 beta chain Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-10475R-APC-Cy7 |
| Product Name | Complement C5 beta chain Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Complement C5 beta chain |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 727 |
| Swiss Prot | P01031 |
| Subcellular location | Secreted |
| Synonyms | Anaphylatoxin C5a; C5; Complement C5; Complement C5 precursor; Complement component 5; CPAMD4; CO5_HUMAN. |
| Background | Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis). |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Complement C5 beta chain Polyclonal Antibody, APC-Cy7 Conjugated (bs-10475R-APC-Cy7) validated for, and at what dilution?
bs-10475R-APC-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Complement C5 beta chain Polyclonal Antibody, APC-Cy7 Conjugated (bs-10475R-APC-Cy7) react with?
Tested reactivity: Human.
How should bs-10475R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Complement C5 beta chain Polyclonal Antibody, APC-Cy7 Conjugated (bs-10475R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human Complement C5 beta chain
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-10475R-APC-Cy7)?
APC-Cy7 on bs-10475R-APC-Cy7: excitation / emission 650nm/780nm.