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Heparanase 2/HPSE2 Polyclonal Antibody, PerCP Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Predicted Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-10485R-PerCP-100uL
Product Name Heparanase 2/HPSE2 Polyclonal Antibody, PerCP Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Predicted Reactivity Human, Mouse, Rat
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Heparanase 2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Secreted, Extracellular matrix
Synonyms heparanase 2; heparanase 3; Heparanase-2; Heparanase 2; heparanase-like protein; Hpa2; HPR2; HPSE2; HPSE2_HUMAN; UFS; Inactive heparanase-2.
Background This gene encodes a heparanase enzyme. The encoded protein is a endoglycosidase that degrades heparin sulfate proteoglycans located on the extracellular matrix and cell surface. This protein may be involved in biological processes involving remodeling of the extracellular matrix including angiogenesis and tumor progression. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009].
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Heparanase 2/HPSE2 Polyclonal Antibody, PerCP Conjugated (bs-10485R-PerCP) validated for, and at what dilution?

bs-10485R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

How should bs-10485R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Heparanase 2/HPSE2 Polyclonal Antibody, PerCP Conjugated (bs-10485R-PerCP)?

KLH conjugated synthetic peptide derived from human Heparanase 2

What are the excitation and emission wavelengths of the PerCP conjugate (bs-10485R-PerCP)?

PerCP on bs-10485R-PerCP: excitation / emission 482nm/677nm.