Connexin-40 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat myocardium tissue labeled with Anti-Connexin-40 Polyclonal Antibody, Unconjugated (bs-1050R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

$Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Connexin 40 Polyclonal Antibody, Unconjugated (bs-1050R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining$

Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Connexin 40 Polyclonal Antibody, Unconjugated (bs-1050R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

$Lane 1: Mouse Kidney Lysates; Lane 2: Rat Kidney Lysates. Probed with Connexin-40 polyclonal Antibody, unconjugated (bs-1050R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Kidney Lysates; Lane 2: Rat Kidney Lysates. Probed with Connexin-40 polyclonal Antibody, unconjugated (bs-1050R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat
Dog

Predicted Reactivity

Cow
Sheep
Pig
Rabbit

Overview
Catalog #	bs-1050R
Product Name	Connexin-40 Polyclonal Antibody
Applications	WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Mouse, Rat, Dog
Predicted Reactivity	Cow, Sheep, Pig, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Connexin-40
Immunogen Range	301-358/358
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2702
Swiss Prot	P36382
Subcellular location	Cell membrane
Synonyms	CX40; Gap junction alpha-5 protein; Connexin-40; GJA5
Background	One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

Predicted Reactivity

Search our catalog of hundreds of thousands of products