TTF1/NKX2-1 Polyclonal Antibody

$Lung lysates probed with TTF1 Polyclonal Antibody, Unconjugated (bs-10539R) at 1:300 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37\u02daC.$

Lung lysates probed with TTF1 Polyclonal Antibody, Unconjugated (bs-10539R) at 1:300 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37\u02daC.

$Lane 1: Human SH-SY5Y cell Lysates; Lane 2: Human HL-60 cell Lysates. Probed with TTF1 polyclonal Antibody, unconjugated (bs-10539R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Human SH-SY5Y cell Lysates; Lane 2: Human HL-60 cell Lysates. Probed with TTF1 polyclonal Antibody, unconjugated (bs-10539R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$Lane 1: Mouse Thyroid gland tissue lysates probed with TTF1 Polyclonal Antibody, Unconjugated (bs-10539R) at 1:200 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse Thyroid gland tissue lysates probed with TTF1 Polyclonal Antibody, Unconjugated (bs-10539R) at 1:200 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(ICC)
IF

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Cow
Sheep
Chicken

Overview
Catalog #	bs-10539r
Product Name	TTF1/NKX2-1 Polyclonal Antibody
Applications	WB ELISA FCM IHC-P IHC-F IF(ICC) IF
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow, Sheep, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TTF1
Immunogen Range	121-220/371
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	7080
Swiss Prot	P43699
Synonyms	thyroid transcription factor1; NK2 homeobox 1; benign chorea; NK-2 homolog A; thyroid nuclear factor; thyroid transcription factor 1; NKX2-1; NKX2.1; BCH; BHC; NK-2; TEBP; TTF1; NKX2A; TITF1; TTF-1; NKX2.1; ZO1_HUMAN.
Background	Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.
Application Dilution
WB
ELISA
FCM
IHC-P
IHC-F
IF(ICC)
IF