IL-1 Alpha Propeptide Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-10540R-Cy5 |
| Product Name | IL-1 Alpha Propeptide Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human IL-1 Alpha Propeptide |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3552 |
| Swiss Prot | P01583 |
| Subcellular location | Secreted |
| Synonyms | interleukin-1 alpha; Hematopoietin 1; IL 1 alpha; IL1 alpha; IL 1; IL 1A; Il-1a; IL1; IL1A; IL1F1; ilia; Interleukin 1 alpha; Interleukin 1 alpha precursor; Interleukin1 alpha; Preinterleukin 1 alpha; Pro interleukin 1 alpha; Prointerleukin 1 alpha; |
| Background | Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is IL-1 Alpha Propeptide Polyclonal Antibody, Cy5 Conjugated (bs-10540R-Cy5) validated for, and at what dilution?
bs-10540R-Cy5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-10540R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IL-1 Alpha Propeptide Polyclonal Antibody, Cy5 Conjugated (bs-10540R-Cy5)?
KLH conjugated synthetic peptide derived from human IL-1 Alpha Propeptide
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-10540R-Cy5)?
Cy5 on bs-10540R-Cy5: excitation / emission 625,650nm/670nm.