Integrin Alpha 3 + Beta 1 Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1057R-BF594-100uL |
| Product Name | Integrin Alpha 3 + Beta 1 Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | Mixed KLH conjugated synthetic peptide derived from human Integrin Alpha 3(976-1025/1051) + Beta 1(35-47/798) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3675, 3688 |
| Swiss Prot | P26006, P05556 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Integrin Alpha 3 / Beta 1; Integrin VLA 4 beta subunit; Alpha 3 subunit of VLA 3 receptor; CD29; CD49C; Fibronectin receptor; Fibronectin receptor beta subunit; Galactoprotein B3; GAPB3; VL3A; VLA 3 alpha chain; VLA3a. |
| Background | alpha3beta1 integrin is a laminin receptor with apparently diverse functions. In epithelial cells it acts as a receptor for the basement membrane, whereas in neuronal and possibly tumor cells it mediates migration. Interactions of alpha3beta1 integrin with tetraspanin proteins may provide clues to how it transduces signals that affect cell behavior. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Integrin Alpha 3 + Beta 1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-1057R-BF594) validated for, and at what dilution?
bs-1057R-BF594 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Integrin Alpha 3 + Beta 1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-1057R-BF594) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse.
How should bs-1057R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Integrin Alpha 3 + Beta 1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-1057R-BF594)?
Mixed KLH conjugated synthetic peptide derived from human Integrin Alpha 3(976-1025/1051) + Beta 1(35-47/798)
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-1057R-BF594)?
AbBy Fluor-594 on bs-1057R-BF594: excitation / emission 590nm/617nm.