CD45 Polyclonal Antibody, PE Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-10599R-PE-100uL |
| Product Name | CD45 Polyclonal Antibody, PE Conjugated |
| Applications |
FCM
|
| Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse CD45 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Swiss Prot | P06800 |
| Subcellular location | Cell membrane |
| Synonyms | Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, Lymphocyte antigen 5, L-CA, Ly-5, T200 |
| Background | Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity. |
| Application Dilution | |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is CD45 Polyclonal Antibody, PE Conjugated (bs-10599R-PE) validated for, and at what dilution?
bs-10599R-PE is validated for FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD45 Polyclonal Antibody, PE Conjugated (bs-10599R-PE) react with?
Tested reactivity: Mouse, Rat.
How should bs-10599R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD45 Polyclonal Antibody, PE Conjugated (bs-10599R-PE)?
KLH conjugated synthetic peptide derived from mouse CD45
What are the excitation and emission wavelengths of the PE conjugate (bs-10599R-PE)?
PE on bs-10599R-PE: excitation / emission 496,564nm/578nm.