CD45 Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-10602r-100uL |
| Product Name | CD45 Polyclonal Antibody |
| Applications |
WB
IHC-F
IF
IHC-P
|
| Reactivity | Mouse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse CD45 |
| Immunogen Range | 131-230/1273 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 19264 |
| Swiss Prot | P06800 |
| Subcellular location | Cell membrane |
| Synonyms | B220; CD45; CD45R; GP180; IMD105; L-CA; LCA; LY5; T200; Ly-5; Lyt-4; loc; RT7; PTPRC_HUMAN; PTPRC; Leukocyte common antigen (L-CA); 3.1.3.48; PTPRC_MOUSE; Lymphocyte antigen 5 (Ly-5); PTPRC_RAT; protein tyrosine phosphatase receptor type C; leukocyte-common antigen; glycoprotein 180 |
| Background | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:500-2000 |
| IHC-F | 1:100-500 |
| IF | 1:100-500 |
| IHC-P | 1:100-500 |
Frequently asked questions
Which applications is CD45 Polyclonal Antibody (bs-10602R) validated for, and at what dilution?
bs-10602R is validated for WB (1:500-2000); IHC-F (1:100-500); IF (1:100-500); IHC-P (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD45 Polyclonal Antibody (bs-10602R) react with?
Tested reactivity: Mouse.
How should bs-10602R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD45 Polyclonal Antibody (bs-10602R)?
KLH conjugated synthetic peptide derived from mouse CD45 Immunogen range: 131-230/1273.