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NME1/NM23A Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-1066R-PE
Product Name NME1/NM23A Polyclonal Antibody, PE Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Nm23-H1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4830
Swiss Prot P15531
Subcellular location Nucleus
Synonyms Nucleoside diphosphate kinase A; NME1; NDP kinase A; NDK A; NM23-H1A; NM23-H1B; NM23-LV; Granzyme A-activated DNase; GAAD; Metastasis inhibition factor nm23; Tumor metastatic process-associated protein; NDPKA; NM23A;
Background NME1 plays a major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. Has tumor metastasis-suppressive capacity.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is NME1/NM23A Polyclonal Antibody, PE Conjugated (bs-1066R-PE) validated for, and at what dilution?

bs-1066R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NME1/NM23A Polyclonal Antibody, PE Conjugated (bs-1066R-PE) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Rabbit.

How should bs-1066R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NME1/NM23A Polyclonal Antibody, PE Conjugated (bs-1066R-PE)?

KLH conjugated synthetic peptide derived from human Nm23-H1

What are the excitation and emission wavelengths of the PE conjugate (bs-1066R-PE)?

PE on bs-1066R-PE: excitation / emission 496,564nm/578nm.