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Podoplanin Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bs-10673R-Cy3
Product Name Podoplanin Polyclonal Antibody, Cy3 Conjugated
Applications
WB
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from Rat Podoplanin
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Swiss Prot Q64294
Subcellular location Cell membrane
Synonyms T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha; Podoplanin; Aggrus; Glycoprotein 38; PA2.26 antigen; Transmembrane glycoprotein E11; Pdpn; Ots8
Background May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is Podoplanin Polyclonal Antibody, Cy3 Conjugated (bs-10673R-Cy3) validated for, and at what dilution?

bs-10673R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Podoplanin Polyclonal Antibody, Cy3 Conjugated (bs-10673R-Cy3) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bs-10673R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Podoplanin Polyclonal Antibody, Cy3 Conjugated (bs-10673R-Cy3)?

KLH conjugated synthetic peptide derived from Rat Podoplanin

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-10673R-Cy3)?

Cy3 on bs-10673R-Cy3: excitation / emission 512,550nm/570,615nm.