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BMP2 Polyclonal Antibody, AbBy Fluor-594 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
  • GuineaPig
Overview
Catalog # bs-10696R-BF594-100uL
Product Name BMP2 Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity Cross-reactive with BMP4
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Horse, Chicken, GuineaPig
Specifications
Conjugation AbBy Fluor-594
Host Rabbit
Source KLH conjugated synthetic peptide derived from human BMP2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 650
Swiss Prot P12643
Subcellular location Secreted
Synonyms bone morphogenetic protein 2; BMP 2; BMP 2A; BMP2; BMP2A; BMP-2; BMP2_HUMAN.
Background BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily of secreted growth factors. It is a disulfide-linked homodimer and induces bone and cartilage formation. In addition to its osteogenic activity, BMP2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. BMPs control fundamental events in early embryonic development, organogenesis and adult tissue homeostasis.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is BMP2 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-10696R-BF594) validated for, and at what dilution?

bs-10696R-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does BMP2 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-10696R-BF594) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Chicken, GuineaPig.

How should bs-10696R-BF594 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise BMP2 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-10696R-BF594)?

KLH conjugated synthetic peptide derived from human BMP2

What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-10696R-BF594)?

AbBy Fluor-594 on bs-10696R-BF594: excitation / emission 590nm/617nm.