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NELL1 Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Cow
  • Sheep
  • Rabbit
Overview
Catalog # bs-10704R-HRP
Product Name NELL1 Polyclonal Antibody, HRP Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Mouse
Predicted Reactivity Human, Rat, Cow, Sheep, Rabbit
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NELL1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4745
Swiss Prot Q92832
Subcellular location Secreted
Synonyms IDH3GL; NEL like protein 1; Nel related protein 1; NEL-like protein 1; Nel-related protein 1; Nell1; NELL1_HUMAN; Neural epidermal growth factor like 1; NRP1; Protein kinase C binding protein NELL1; Protein kinase C-binding protein NELL1.
Background This gene encodes a cytoplasmic protein that contains epidermal growth factor (EGF)-like repeats. The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation. A similar protein in rodents is involved in craniosynostosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is NELL1 Polyclonal Antibody, HRP Conjugated (bs-10704R-HRP) validated for, and at what dilution?

bs-10704R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NELL1 Polyclonal Antibody, HRP Conjugated (bs-10704R-HRP) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Sheep, Rabbit.

How should bs-10704R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NELL1 Polyclonal Antibody, HRP Conjugated (bs-10704R-HRP)?

KLH conjugated synthetic peptide derived from human NELL1