HRAS Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1071R-APC-Cy7-100uL |
| Product Name | HRAS Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Pig |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human H-ras |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3265 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | c bas/has; c H ras; c has/bas p21 protein; C K RAS; c K ras2 protein; c Ki ras; c Kirsten ras protein; c ras Ki 2 protein; Cellular c Ki ras2 proto oncogene antibody G1III6 N ras; GTPase HRas; GTPase KRas; GTPase NRas; H Ras 1; H RasIDX; Ha Ras; HRAS 1; HRAS; HRAS1; K Ras 2; K ras; K ras p21 protein; K RAS2A; K RAS2B; K RAS4A; K RAS4B; KI RAS; Kirsten rat sarcoma 2 viral v Ki ras2 oncogene homolog; KRAS 1; KRAS 2; KRAS; KRAS1; KRAS2; N ras; N ras oncogene; Neuroblastoma RAS viral v ras oncogene homolog; NRAS 1; NRAS; NRAS1; NS3; Oncogene KRAS2; p21ras; PR310 c K ras oncogene antibody RASH 1; RASH1; RASK 2; RASK2; Transforming protein N Ras; Transforming protein p21; v Ha ras Harvey rat sarcoma viral oncogene homolog; v Ki ras2 Kirsten rat sarcoma 2 viral oncogene homolog; v Ki ras2 Kirsten rat sarcoma viral oncogene homolog; v ras neuroblastoma RAS viral oncogene homolog; RASH_HUMAN. |
| Background | This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HRAS Polyclonal Antibody, APC-Cy7 Conjugated (bs-1071R-APC-Cy7) validated for, and at what dilution?
bs-1071R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HRAS Polyclonal Antibody, APC-Cy7 Conjugated (bs-1071R-APC-Cy7) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Pig.
How should bs-1071R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HRAS Polyclonal Antibody, APC-Cy7 Conjugated (bs-1071R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human H-ras
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-1071R-APC-Cy7)?
APC-Cy7 on bs-1071R-APC-Cy7: excitation / emission 650nm/780nm.