Staphylococcus Enterotoxin B antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-10722R-RBITC |
| Product Name | Staphylococcus Enterotoxin B antibody, RBITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Bacteria |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Staphylococcus Enterotoxin B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Secreted |
| Background | Staphylococcal enterotoxins represent a group of proteins, which are secreted by Staphylococcus aureus and cause staphylococcal food poisoning syndrome. The illness is characterised by high fever, hypotension, diarrhea, shock, and in some cases death. Their molecular masses range between 27 and 30 kDa. At present, seven enterotoxins are known, namely A, B, C1, C2, C3, D and E. Their amino acid sequences have been determined and it was shown that all are single chain polypeptides containing one disulfide bond formed by two half cystines located in the middle of the polypeptide chain, which form the so called cysteine loop. SEB is an extremely potent activator of T cells, stimulating the production and secretion of various cytokines which mediate many of the toxic effects of SEB. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Staphylococcus Enterotoxin B antibody, RBITC Conjugated (bs-10722R-RBITC) validated for, and at what dilution?
bs-10722R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Staphylococcus Enterotoxin B antibody, RBITC Conjugated (bs-10722R-RBITC) react with?
Tested reactivity: Bacteria.
How should bs-10722R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Staphylococcus Enterotoxin B antibody, RBITC Conjugated (bs-10722R-RBITC)?
KLH conjugated synthetic peptide derived from Staphylococcus Enterotoxin B
What are the excitation and emission wavelengths of the RBITC conjugate (bs-10722R-RBITC)?
RBITC on bs-10722R-RBITC: excitation / emission 540nm/625nm.