RRAGA + RRAGB Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10731R-BF555 |
| Product Name | RRAGA + RRAGB Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RRAGA and RRAGB |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10670 |
| Swiss Prot | Q7L523 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | FIP1; RAGA; FIP-1; RRAGB; Adenovirus E3 14.7 kDa-interacting protein 1; Rag A; RAg B; RagA; RAGB; Ras related GTP binding protein A; RRAGA_HUMAN; Ras-related GTP binding A? GTP-binding protein ragB; Rag B; RagB; Ras-related GTP-binding protein B; RRAGB; RRAGB_HUMAN.. |
| Background | Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is RRAGA + RRAGB Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-10731R-BF555) validated for, and at what dilution?
bs-10731R-BF555 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RRAGA + RRAGB Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-10731R-BF555) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Sheep, Pig, Horse.
How should bs-10731R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RRAGA + RRAGB Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-10731R-BF555)?
KLH conjugated synthetic peptide derived from human RRAGA and RRAGB
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-10731R-BF555)?
AbBy Fluor-555 on bs-10731R-BF555: excitation / emission 553nm/568nm.