Alpha neoendorphin Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Alpha neoendorphin) Polyclonal Antibody, Unconjugated (bs-10741R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Alpha neoendorphin) Polyclonal Antibody, Unconjugated (bs-10741R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Sheep
Pig
Horse
Rabbit
Guinea Pig

Overview
Catalog #	bs-10740r
Product Name	Alpha neoendorphin Polyclonal Antibody
Applications	IHC-P IHC-F IF
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit, Guinea Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Alpha neoendorphin
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5173
Swiss Prot	P01213
Subcellular location	Cytoplasm
Synonyms	prodynorphin; proenkephalin-B; Alpha neoendorphin; Alpha-neoendorphin; PDYN_HUMAN.
Application Dilution
IHC-P	=1:100-500
IHC-F	IHC-F=1:100-500
IF	IF=1:100-500