Caspase-1 p20 Polyclonal Antibody, PE Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-10743R-PE |
| Product Name | Caspase-1 p20 Polyclonal Antibody, PE Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Caspase-1 p20 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 834 |
| Swiss Prot | P29466 |
| Subcellular location | Cytoplasm |
| Synonyms | Caspase-1 subunit p20; Caspase-1; CASP-1; IL-1 beta-converting enzyme; Interleukin-1 beta-converting enzyme; IL1BC; IL1BCE; CASP1 |
| Background | Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is Caspase-1 p20 Polyclonal Antibody, PE Conjugated (bs-10743R-PE) validated for, and at what dilution?
bs-10743R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Caspase-1 p20 Polyclonal Antibody, PE Conjugated (bs-10743R-PE) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-10743R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Caspase-1 p20 Polyclonal Antibody, PE Conjugated (bs-10743R-PE)?
KLH conjugated synthetic peptide derived from human Caspase-1 p20
What are the excitation and emission wavelengths of the PE conjugate (bs-10743R-PE)?
PE on bs-10743R-PE: excitation / emission 496,564nm/578nm.