SIGLEC1 Polyclonal Antibody

Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-Sialoadhesin Polyclonal Antibody, Unconjugated (bs-10751R) at 1:300 followed by conjugation to the secondary antibody (bs-0295G-HRP) and DAB staining.

Formalin-fixed and paraffin embedded rat spleen labeled with Anti-Sialoadhesin Polyclonal Antibody, Unconjugated (bs-10751R) at 1:500 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat lung labeled with Anti-Sialoadhesin Polyclonal Antibody, Unconjugated (bs-10751R) at 1:500 followed by conjugation to the secondary antibody and DAB staining

$U937 cells probed with Rabbit Anti-Sialoadhesin Polyclonal Antibody, ALEXA FLUOR\u00ae 647 Conjugated (bs-10751R-A647) for 30 minutes compared to control cells (blue) and isotype control (orange).$

U937 cells probed with Rabbit Anti-Sialoadhesin Polyclonal Antibody, ALEXA FLUOR\u00ae 647 Conjugated (bs-10751R-A647) for 30 minutes compared to control cells (blue) and isotype control (orange).

Applications

ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Rat

Overview
Catalog #	bs-10751R
Product Name	SIGLEC1 Polyclonal Antibody
Applications	ELISA FCM IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Sialoadhesin
Immunogen Range	851-950/1709
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6614
Swiss Prot	Q9BZZ2
Subcellular location	Secreted, Cell membrane
Synonyms	SN; CD169; SIGLEC-1; dJ1009E24.1; Sialoadhesin; Sialic acid-binding Ig-like lectin 1; SIGLEC1
Background	Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis.
Application Dilution
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200