SIGLEC1 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-10751R-BF488 |
| Product Name | SIGLEC1 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Sialoadhesin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6614 |
| Swiss Prot | Q9BZZ2 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | SN; CD169; SIGLEC-1; dJ1009E24.1; Sialoadhesin; Sialic acid-binding Ig-like lectin 1; SIGLEC1 |
| Background | Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SIGLEC1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-10751R-BF488) validated for, and at what dilution?
bs-10751R-BF488 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SIGLEC1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-10751R-BF488) react with?
Tested reactivity: Human, Rat.
How should bs-10751R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SIGLEC1 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-10751R-BF488)?
KLH conjugated synthetic peptide derived from human Sialoadhesin
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-10751R-BF488)?
AbBy Fluor-488 on bs-10751R-BF488: excitation / emission 499nm/519nm.