Secretogranin V Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10762R-PE-Cy5 |
| Product Name | Secretogranin V Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Secretogranin-5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6447 |
| Swiss Prot | P05408 |
| Subcellular location | Secreted |
| Synonyms | 7B2; SgV; P7B2; SGNE1; Neuroendocrine protein 7B2; Pituitary polypeptide; Secretogranin V; Secretogranin-5; Secretory granule endocrine protein I; SCG5 |
| Background | Acts as a molecular chaperone for PCSK2/PC2, preventing its premature activation in the regulated secretory pathway. Binds to inactive PCSK2 in the endoplasmic reticulum and facilitates its transport from there to later compartments of the secretory pathway where it is proteolytically matured and activated. Also required for cleavage of PCSK2 but does not appear to be involved in its folding. Plays a role in regulating pituitary hormone secretion. The C-terminal peptide inhibits PCSK2 in vitro. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Secretogranin V Polyclonal Antibody, PE-Cy5 Conjugated (bs-10762R-PE-Cy5) validated for, and at what dilution?
bs-10762R-PE-Cy5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Secretogranin V Polyclonal Antibody, PE-Cy5 Conjugated (bs-10762R-PE-Cy5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-10762R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Secretogranin V Polyclonal Antibody, PE-Cy5 Conjugated (bs-10762R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human Secretogranin-5
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-10762R-PE-Cy5)?
PE-Cy5 on bs-10762R-PE-Cy5: excitation / emission 488nm/670nm.