DHRS2 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-10771R-BF488 |
| Product Name | DHRS2 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DHRS2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10202 |
| Swiss Prot | Q13268 |
| Subcellular location | Nucleus |
| Synonyms | HEP27; SDR25C1; Dehydrogenase/reductase SDR family member 2, mitochondrial; Dicarbonyl reductase HEP27; Protein D; DHRS2 |
| Background | Displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates. No reductase activity is displayed in vitro with steroids, retinoids and sugars as substrates. Attenuates MDM2-mediated p53/TP53 degradation, leading to p53/TP53 stabilization and increased transcription activity, resulting in the accumulation of MDM2 and CDKN1A/p21. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DHRS2 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-10771R-BF488) validated for, and at what dilution?
bs-10771R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DHRS2 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-10771R-BF488) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-10771R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DHRS2 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-10771R-BF488)?
KLH conjugated synthetic peptide derived from human DHRS2
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-10771R-BF488)?
AbBy Fluor-488 on bs-10771R-BF488: excitation / emission 499nm/519nm.