NGFB Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10806R-APC-Cy5.5 |
| Product Name | NGFB Polyclonal Antibody, APC-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Rabbit |
| Source | Recombinded human NGF beta full length protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4803 |
| Swiss Prot | P01138 |
| Subcellular location | Secreted |
| Synonyms | NGF; Beta nerve growth factor; NGF-beta; Beta nerve growth factor precursor; Beta NGF; HSAN5; MGC161426; MGC161428; Nerve growth factor beta; Nerve growth factor beta polypeptide; Nerve growth factor beta subunit; NGF B; NGFB; NID67; NGF beta; NGF-B; NGF_HUMAN. |
| Background | Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NGFB Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-10806R-APC-CY5-5) validated for, and at what dilution?
BS-10806R-APC-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NGFB Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-10806R-APC-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig.
How should BS-10806R-APC-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NGFB Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-10806R-APC-CY5-5)?
Recombinded human NGF beta full length protein
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-10806R-APC-CY5-5)?
APC-Cy5.5 on BS-10806R-APC-CY5-5: excitation / emission 650nm/694nm.