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NGFB Polyclonal Antibody, AbBy Fluor-488 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
Overview
Catalog # bs-10806R-BF488
Product Name NGFB Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig
Specifications
Conjugation AbBy Fluor-488
Host Rabbit
Source Recombinded human NGF beta full length protein
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4803
Swiss Prot P01138
Subcellular location Secreted
Synonyms NGF; Beta nerve growth factor; NGF-beta; Beta nerve growth factor precursor; Beta NGF; HSAN5; MGC161426; MGC161428; Nerve growth factor beta; Nerve growth factor beta polypeptide; Nerve growth factor beta subunit; NGF B; NGFB; NID67; NGF beta; NGF-B; NGF_HUMAN.
Background Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is NGFB Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-10806R-BF488) validated for, and at what dilution?

bs-10806R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NGFB Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-10806R-BF488) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig.

How should bs-10806R-BF488 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NGFB Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-10806R-BF488)?

Recombinded human NGF beta full length protein

What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-10806R-BF488)?

AbBy Fluor-488 on bs-10806R-BF488: excitation / emission 499nm/519nm.