GRK1 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded mouse lung labeled with Anti-GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Lane 1: Human HUVEC cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human Ramos cell lysates probed with GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HUVEC cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human Ramos cell lysates probed with GRK1 Polyclonal Antibody, Unconjugated (bs-1082R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Cow
Chicken

Overview
Catalog #	bs-1082R
Product Name	GRK1 Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GRK1
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6011
Swiss Prot	Q15835
Subcellular location	Cell membrane
Synonyms	G-protein coupled receptor kinase 1; GRK1; GPRK1; RK; Grk1; Rhok; RHODOPSIN KINASE; RK_HUMAN.
Application Dilution
WB	=1:500-2000
IHC-P	ELISAELISA=1:5000-10000
IHC-F
IF