phospho-Ephrin B2 (Tyr311/316) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10940R-Cy3 |
| Product Name | phospho-Ephrin B2 (Tyr311/316) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated Synthesised phosphopeptide derived from human Ephrin B2 around the phosphorylation site of Tyr311/316 |
| Modification Site | Tyr311/316 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1947 |
| Swiss Prot | P52799 |
| Subcellular location | Cell membrane |
| Synonyms | phospho-Ephrin B2 (Tyr311+Tyr316); Ephrin B2 (phospho Y311 + Y316); CEK5 ligand; EFNB1; ELK L; EphrinB; Ephrin-B; ELK ligand; ELKL; EPH related receptor tyrosine kinase ligand 2; Ephrin B Precursor; Ephrin B1; Ephrin B2; LERK 2; LERK2; EFNB1_HUMAN; EFNB2_HUMAN. |
| Application Dilution | |
| WB | =1:200-1000 |
Frequently asked questions
Which applications is phospho-Ephrin B2 (Tyr311/316) Polyclonal Antibody, Cy3 Conjugated (bs-10940R-Cy3) validated for, and at what dilution?
bs-10940R-Cy3 is validated for WB (WB=1:200-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Ephrin B2 (Tyr311/316) Polyclonal Antibody, Cy3 Conjugated (bs-10940R-Cy3) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-10940R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Ephrin B2 (Tyr311/316) Polyclonal Antibody, Cy3 Conjugated (bs-10940R-Cy3)?
KLH conjugated Synthesised phosphopeptide derived from human Ephrin B2 around the phosphorylation site of Tyr311/316
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-10940R-Cy3)?
Cy3 on bs-10940R-Cy3: excitation / emission 512,550nm/570,615nm.