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PDGFRA Polyclonal Antibody, AbBy Fluor-750 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Horse
Overview
Catalog # bs-10989R-BF750-100uL
Product Name PDGFRA Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Sheep, Horse
Specifications
Conjugation AbBy Fluor-750
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PDGFRA
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5156
Swiss Prot P16234
Subcellular location Cell membrane
Synonyms PDGF Receptor alpha; Plateletdirived growth factor receptor-alpha; Alpha platelet derived growth factor receptor; CD 140a; CD140a; CD140a antigen; MGC74795; PDGF alpha chain; PDGF R alpha; PDGFR 2; PDGFR A; PDGFR alpha; PDGFR2; PDGFRA; Platelet derived growth factor receptor 2; Platelet derived growth factor receptor alpha; Platelet derived growth factor receptor alpha polypeptide; PGFRA_HUMAN.
Background This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers. [provided by RefSeq, Mar 2012]
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is PDGFRA Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-10989R-BF750) validated for, and at what dilution?

bs-10989R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PDGFRA Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-10989R-BF750) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Horse.

How should bs-10989R-BF750 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PDGFRA Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-10989R-BF750)?

KLH conjugated synthetic peptide derived from human PDGFRA

What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-10989R-BF750)?

AbBy Fluor-750 on bs-10989R-BF750: excitation / emission 749nm/775nm.

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