FAM84A Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11010R-PerCP-100uL |
| Product Name | FAM84A Polyclonal Antibody, PerCP Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Pig, Chicken, Zebrafish |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NSE1/FAM84A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3859 |
| Synonyms | FAM 84A; Family with sequence similarity 84 member A; family with sequence similarity 84, member A; FLJ35392; Neurologic sensory protein 1; Neurological/sensory 1; NSE 1; NSE1; PP11517; Protein FAM84A; Protein NSE1. |
| Background | FAM84A is a 292 amino acid protein that belongs to the FAM84 family of proteins. Predominantly expressed in testis, FAM84A shares 44% amino acid identity with the related protein FAM84B. FAM84A localizes to a subcellular membrane region where there is no contact between neighboring cells and is believed to play a role in cell morphology and motility. More specifically, the expression of FAM84A increases cell motility. Two FAM84A isoforms are expressed due to alternative splicing events. Isoform 2 can be phosphorylated on various serine residues and this phosphorylation is associated with cellular morphology. FAM84A is upregulated in colorectal cancer, lung cancer, pancreatic cancer, cholangiocarcinoma and bladder cancer tissues. Via its ability to increase cell motility, FAM84A may contribute to the invasion and metastasis of cancer cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is FAM84A Polyclonal Antibody, PerCP Conjugated (bs-11010R-PerCP) validated for, and at what dilution?
bs-11010R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FAM84A Polyclonal Antibody, PerCP Conjugated (bs-11010R-PerCP) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Chicken, Zebrafish.
How should bs-11010R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FAM84A Polyclonal Antibody, PerCP Conjugated (bs-11010R-PerCP)?
KLH conjugated synthetic peptide derived from human NSE1/FAM84A
What are the excitation and emission wavelengths of the PerCP conjugate (bs-11010R-PerCP)?
PerCP on bs-11010R-PerCP: excitation / emission 482nm/677nm.