B7-H3/CD276 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11019R-BF750-100uL |
| Product Name | B7-H3/CD276 Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD276/B7H3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 80381 |
| Swiss Prot | Q5ZPR3 |
| Subcellular location | Cell membrane |
| Synonyms | B7H3; B7-H3; B7RP-2; 4Ig-B7-H3; CD276 antigen; B7 homolog 3; Costimulatory molecule; CD276; PSEC0249; UNQ309/PRO352 |
| Background | May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is B7-H3/CD276 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11019R-BF750) validated for, and at what dilution?
bs-11019R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does B7-H3/CD276 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11019R-BF750) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Pig, Horse.
How should bs-11019R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise B7-H3/CD276 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-11019R-BF750)?
KLH conjugated synthetic peptide derived from human CD276/B7H3
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-11019R-BF750)?
AbBy Fluor-750 on bs-11019R-BF750: excitation / emission 749nm/775nm.