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MAP1B Polyclonal Antibody, AbBy Fluor-594 Conjugated

Applications

  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-11028R-BF594-100uL
Product Name MAP1B Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation AbBy Fluor-594
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MAP1B
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4131
Swiss Prot P46821
Subcellular location Cytoplasm, Cell membrane
Synonyms MAP5; FUTSCH; PPP1R102; Microtubule-associated protein 1B; MAP-1B; MAP1B
Background Facilitates tyrosination of alpha-tubulin in neuronal microtubules (By similarity). Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules. Acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation and membrane blebbing.
Application Dilution
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is MAP1B Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-11028R-BF594) validated for, and at what dilution?

bs-11028R-BF594 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MAP1B Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-11028R-BF594) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-11028R-BF594 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MAP1B Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-11028R-BF594)?

KLH conjugated synthetic peptide derived from human MAP1B

What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-11028R-BF594)?

AbBy Fluor-594 on bs-11028R-BF594: excitation / emission 590nm/617nm.