OTOA Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11060R-PerCP-Cy5.5-100uL |
| Product Name | OTOA Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human OTOA/DFNB22 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 146183 |
| Swiss Prot | Q7RTW8 |
| Subcellular location | Secreted, Cell membrane, Extracellular matrix |
| Synonyms | Cancer/testis antigen 108; CT108; Deafness, autosomal recessive 22; DFNB22; OTOA; OTOAN_HUMAN; Otoancorin. |
| Background | Otoancorin, also known as OTOA, CT108 or DFNB22, is a 1,153 amino acid protein belonging to the stereocilin family. Expressed in the inner ear and restricted to the interface between the apical surface of sensory epithelia, otoancorin is suggested to act as an adhesion molecule. Otoancorin ensures the attachment of the inner ear acellular gels to the apical surface of the underlying nonsensory cells. Mutations in the gene encoding otoancorin leads to deafness autosomal recessive type 22 (DFNB22), which is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain or the area of the brain that receives sound information. Existing as three alternatively spliced isoforms, otoancorin is encoded by a gene located on human chromosome 16p12.2. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is OTOA Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-11060R-PERCP-CY5-5) validated for, and at what dilution?
BS-11060R-PERCP-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does OTOA Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-11060R-PERCP-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat.
How should BS-11060R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise OTOA Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-11060R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from human OTOA/DFNB22
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-11060R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-11060R-PERCP-CY5-5: excitation / emission 482nm/694nm.