Neurotrimin Polyclonal Antibody

$Human Huvec, HepG2, and Jurkat cells probed with Neurotrimin Polyclonal Antibody, unconjugated (bs-11082R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.$

Human Huvec, HepG2, and Jurkat cells probed with Neurotrimin Polyclonal Antibody, unconjugated (bs-11082R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

$Mouse brain lysates probed with Neurotrimin Polyclonal Antibody, unconjugated (bs-11082R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.$

Mouse brain lysates probed with Neurotrimin Polyclonal Antibody, unconjugated (bs-11082R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

$Paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Neurotrimin Polyclonal Antibody, Unconjugated (bs-11082R) at 1:300 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining$

Paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Neurotrimin Polyclonal Antibody, Unconjugated (bs-11082R) at 1:300 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Predicted Reactivity

Human
Mouse
Rat
Dog
Sheep
Pig
Horse
Rabbit

Overview
Catalog #	bs-11082R
Product Name	Neurotrimin Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Predicted Reactivity	Human, Mouse, Rat, Dog, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Neurotrimin/HNT
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	50863
Swiss Prot	Q9P121
Subcellular location	Cell membrane
Synonyms	hNT; IgLON family member 2; IGLON2; Neurotrimin; NT antibody NTM; NTRI_HUMAN.
Background	Cell adhesion molecules (CAMs) influence cell growth, differentiation, embryogenesis, immune response and cancer metastasis by networking information from the extracellular matrix to the cell. The four major families of cell adhesion molecules are immunoglobulin (Ig) superfamily (calcium-independent transmembrane glycoproteins), integrins (transmembrane non-covalently linked heterodimers of Alpha and Beta subunits), calcium-dependent cadherins and divalent cation-dependent selectins. Regulation of neuronal synaptic adhesion by CAMs has proven important for learning and memory. Proper embryonic morphogenic development is also heavily dependent on the regulation of cell adhesion molecules. Neurotrimin (hNT) is a neural cell adhesion molecule localizing to the cell membrane, where it acts as a lipid-anchor. Neurotrimin belongs to the IgLON family of proteins, a member of the larger immunoglobulin superfamily.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200