NCAM2 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11094R-BF350-100uL |
| Product Name | NCAM2 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NCAM2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | N CAM 2; N CAM 21; N CAM2; N CAM21; N-CAM-2; NCAM 2; NCAM 21; NCAM-2; Ncam2; NCAM2_HUMAN; NCAM21; Neural cell adhesion molecule 2; OCAM GPI; R4B12; RB 8 neural cell adhesion molecule; RB8 neural cell adhesion molecule; RNCAM. |
| Background | NCAM2 is an 837 amino acid protein encoded by the human gene NCAM2. NCAM2 contains five immunoglobulin-like domains, two Fibronectin type III domains, a transmembrane domain and a cytoplasmic domain. The gene is expressed most strongly in human adult and fetal brain. NCAM2 is a member of the neural cell adhesion molecule (NCAM) family. NCAMs are closely related cell surface glycoproteins involved in cell to cell interactions during growth and are thought to play an important role in embryogenesis and development. NCAM2 is a considered a good candidate for involvement in certain Down syndrome phenotypes because a slight overexpression of NCAMs increases many-fold the homotypic adhesion properties of cells. Stat5 regulates NCAM2 in vivo by binding to the NCAM2 intron in the NKL natural killer cell line; this binding is induced by cytokines that activate Stat5. Neither Stat1 nor Stat3 bind to this region, despite sharing a consensus binding sequence with Stat5. |
| Application Dilution | |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is NCAM2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-11094R-BF350) validated for, and at what dilution?
bs-11094R-BF350 is validated for FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NCAM2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-11094R-BF350) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Rabbit.
How should bs-11094R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NCAM2 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-11094R-BF350)?
KLH conjugated synthetic peptide derived from human NCAM2
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-11094R-BF350)?
AbBy Fluor-350 on bs-11094R-BF350: excitation / emission 346nm/442nm.