NIPAL3 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-11097R-BF680 |
| Product Name | NIPAL3 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NIPAL3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cell membrane |
| Synonyms | NPAL3; NPAL-3; NIPA like domain containing 3; NIPA like protein 3; RGD1563439; RP23-332E2.5; RP3-462O23.3; NPAL3_HUMAN. |
| Background | NIPAL3 is a 406 amino acid multi-pass membrane protein that belongs to the NIPA family and exists as three alternatively spliced isoforms. The gene that encodes NPAL3 consists of approximately 57,229 bases and maps to human chromosome 1p36. Chromosome 1 is the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome. There are about 3,000 genes on chromosome 1, and considering the great number of genes there are also a large number of diseases associated with chromosome 1. Notably, the rare aging disease Hutchinson-Gilford progeria is associated with the LMNA gene which encodes lamin A. When defective, the LMNA gene product can build up in the nucleus and cause characteristic nuclear blebs. The MUTYH gene is located on chromosome 1 and is partially responsible for familial adenomatous polyposis. Stickler syndrome, Parkinsons, Gaucher disease and Usher syndrome are also associated with chromosome 1. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NIPAL3 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-11097R-BF680) validated for, and at what dilution?
bs-11097R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NIPAL3 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-11097R-BF680) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-11097R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NIPAL3 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-11097R-BF680)?
KLH conjugated synthetic peptide derived from human NIPAL3
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-11097R-BF680)?
AbBy Fluor-680 on bs-11097R-BF680: excitation / emission 679nm/702nm.